Web10 jun. 2014 · Myc tag – This tag is a short peptide sequence (EQKLISEEDL ) derived from the c-myc gene product and recognized by numerous commercial antibodies. It can be added to a protein using … WebTandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions. The goal is to extract from a cell only the protein …

Anti-C-Myc Magnetic Beads - Sigma-Aldrich

Web22 mei 2024 · 3.2 Purification of the c-Myc b-HLH-LZ 1. Take the suspended pellets from −80 °C and immerse in hot tap water (50–60°C). 2. Once the suspension is thawed, freeze the homogenate with liquid nitrogen then thaw in hot water ( see Note 4 ). Repeat this freeze–thaw cycle until the suspension is at maximum viscosity (3–4 cycles). 3. WebRecombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an N-terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous and contains no detectable protease, DNase and RNase activity. References chuck e cheese birthday 2018

Myc-tagged protein purification - Takara Bio

WebHere, we provide methods to produce and purify high quantities of pure and untagged b-HLH-LZ constructs of c-Myc and Max as well as specific c-Myc/Max heterodimers for … WebA purification protocol was devised which allowed the c-myc protein to be readily purified in quantities sufficient for detailed biochemical and physical analyses. A high-titer polyclonal antiserum was raised against the pure protein and shown to immunoprecipitate the p110gag-myc fusion protein of MC-29-infected quail cells. chuck e cheese birthday 2009